Microplate Readers and Reader Accessories

Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.

The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.

In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.

Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.

Features may include:

Monochromator- or filter-based wavelength selection
Visible only or UV/Vis spectrum, fluorescence, and luminescence
Multiple photomultiplier tubes (PMTs) for simultaneous assays
Temperature and CO₂/O₂controls
Modular design for adding functionality

Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.

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286

Revvity Health Sciences Inc HTRF High Performance Human IL-6 Detection Kit, Standard, 1 vial

HTRF High Performance Human IL-6 Detection Kit, Standard, 1 vial

Pricing & Availability

287

Revvity Health Sciences Inc HTRF Human & Mouse Total BRD4 Detection Kit, Control Lysate

HTRF Human & Mouse Total BRD4 Detection Kit, Control Lysate

Pricing & Availability

288

Revvity Health Sciences Inc HTRF Human LAG3 / MHCII Binding Kit, 500 Assay Points

HTRF Human LAG3 / MHCII Binding Kit, 500 Assay Points

Pricing & Availability

289

Molecular Devices Fluorescence Polarization Filter, 635 nm, Emission

Fluorescence polarization emission filter centered at 635 nm for use with SpectraMax iD5 microplate readers. The filter is intended for fluorescence polarization assays and mounts in the instrument filter slide for straightforward exchange.