Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Emission Filter, Time-Resolved, 665 nm, 10 nm
Molecular Devices time-resolved acceptor emission filter supplied as a filter slide with holder. It provides a 665 nm center wavelength with a 10 nm bandwidth for sensitive acceptor emission detection in time-resolved fluorescence assays and is designed for use in compatible multi-mode microplate readers.
- Center wavelength 665 nm.
- Bandwidth 10 nm.
- Filter type time-resolved acceptor emission.
- Form factor filter slide with holder.
- Intended applications TR-FRET and HTRF assays.
- Compatible with multi-mode microplate readers that accept filter slides.
Electron Microscopy Sciences NIGHTSEA™ Fluorescence Adapter VI Light Head, Ultra Violet
Additional combinations excitation/ emission wavelength combination can be added NIGHTSEA™ Stereo Microscope Fluorescence Viewing system by purchasing individual light head, barrier filter, and viewing shield combinations. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.
Color: Ultra Violet
Excitation: 360-380 nm
Emission filter: 415 nm longpass
Fluorophores: DAPI
Revvity Health Sciences Inc HTRF Human Total HPK1 Detection Kit, 50,000 Assay Points
HTRF Human Total HPK1 Detection Kit, 50,000 Assay Points
Molecular Devices Membrane Holder, Western blot, For Microplate Reader
The ScanLater membrane holder secures western blot membranes for scanning within the ScanLater western blot detection workflow on compatible multi-mode microplate readers. It positions membranes for reproducible scanning and integrates with membrane detection modules to enable time-resolved fluorescence measurements.
- Holds standard western blot membranes securely for scanning.
- Positions membranes reproducibly to improve scan consistency.
- Integrates into a microplate reader workflow for membrane detection.
- Enables time-resolved fluorescence membrane measurements when used with compatible detection hardware.
- Simple insertion and removal for efficient sample handling.
Revvity Health Sciences Inc HTRF Human Phospho-JAK2 (Tyr1007/1008) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-JAK2 (Tyr1007/1008) Detection Kit, 10,000 Assay Points
Electron Microscopy Sciences MultiLap™ Fish Otolith Polishing Tool 6-Position Alignment Fixture
For use with Model 195 - 6 Position MultiLap (50168-95).
Revvity Health Sciences Inc HTRF Strep-Tactin Eu-Conjugate, 20,000 Assay Points
HTRF Strep-Tactin Eu-Conjugate, 20,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, Control Lysate
HTRF Human Phospho-ATM (Ser1981) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
Molecular Devices Luminescence Filter, Long-pass 610 nm, Emission
The Molecular Devices 610 nm LP luminescence filter (cat. #6590-0117) is a long-pass emission filter optimized for luminescence detection and BRET acceptor measurements. It is intended for use as an acceptor/emission filter in NanoBRET assays and is documented for compatibility with filter-based multi-mode microplate readers such as the SpectraMax iD5.
- Provides a long-pass emission cutoff at 610 nm.
- Optimized for luminescence and BRET acceptor detection.
- Designed for use in filter-based microplate reader optical systems.
- Supports assays requiring acceptor emission separation in NanoBRET workflows.
Molecular Devices Fluorescence Polarization Filter Set, 485 nm, Polarized
Molecular Devices set of two fluorescence polarization filters designed for fluorescence polarization assays. The matched vertical and horizontal polarized filters have a 485 nm center wavelength and are intended for compatible multi-mode microplate readers. Manufacturer documents report filter bandwidth as either 20 nm or 25 nm.
- Set of two polarized filters supplied as a matched vertical and horizontal pair.
- Center wavelength 485 nm for excitation in fluorescence polarization assays.
- Bandpass bandwidth reported as 20-25 nm across manufacturer documents.
- Designed for use with compatible multi-mode microplate readers to enable FP measurements.
- Supplied as accessory filters for configuring microplate readers for FP assays.
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 500 Assay Points
HTRF Human Phospho-ATM (Ser1981) Detection Kit, 500 Assay Points